| Jean-Philippe
Hugnot |
 |
Function
: Assitant Professor University Montpellier 2
Academic and research
track :
JP Hugnot started his scientic career as a PhD student in
1989 in Dr Axel Kahn’s lab at the Cochin Hospital in
Paris. He worked on the characterization of the Duchenne
Myopathy Dystrophy gene. He did his first postdoc in 1993
in Pr Michel Lazdunski’s lab in Nice on the cloning
and characterization of new K+ channels. He then spent 2
years at the Institute of Psychiatry in London to work on
the nervous system development and neural stem cells
with John Sinden and Jack Price. When he returned to France
in 2000 he set up his research group focusing on spinal cord
neural stem cells in A Privat’s team in Montpellier.
JP Hugnot is currently a permanent lecturer in basic cellular
biology and neurobiology (developmental neurobiology, neuroanatomy,
neurophysiology) for Licence and Master students at the Montpellier
University.
Main achievements
:
-Cloning of a new family of silent K+ channel subunits acting
as modulatory subunits of Kv2 channels.
-Cloning of new members of protein-G-activated K+ channels.
-Showing the key role of endogenous cytokines in the self-renewal
and differentiation of neural stem cells.
-Characterization of human/rodent ependymal neural stem cells
niche and isolation of nestin+ sox2+ neural stem cells from
the adult human spinal cord using post mortem tissues.
Research interests
:
-investigating the role of neural stem/progenitor cells
in the adult rodent normal central nervous system, especially
spinal cord, notably using transgenic mouse approaches.
-exploring the presence of neural stem/progenitor cells in
the adult human central nervous system.
-identifying key molecular mechanisms involved in the formation
of nervous system cellular diversity from neural stem cells,
especially asymmetric division and endogenous cytokines.
To this end, neural stem cells cultures derived from normal
or tumoral tissues (glioma) from human and rodents are used.
-exploring new therapeuric strategies for treating spinal
cord diseases (traumatic lesions and degenerative diseases)
based on the use of endogenous or exogenous neural stem/progenitor
cells .
Selected publications
:
Adult human spinal cord harbours neural
precursor cells that generate neurons and glial cells in
vitro. C. Dromard, H. Guillon, V. Rigau, C. Ripoll, S Bozza,
P. Sabatier, N Lonjon, H Duffau, F. Vachiery-Lahaye, M. Prieto,
C Tran Van Ba, L. Deleyrolle, A. Boularan, K. Langley, M.
Gaviria, A. Privat, L. Bauchet*, J.P. Hugnot*, J
Neurosci Res, in press (*= co auteurs)
Dromard C, Bartolami S, Deleyrolle L, Takebayashi
H, Ripoll C, Simonneau L,Prome S, Puech S, Tran VB, Duperray
C, Valmier J, Privat A, Hugnot JP.
NG2 and Olig2 expression provides evidence for phenotypic deregulation
of
cultured central nervous system and peripheral nervous system
neural precursor
cells. Stem Cells. 2007 Feb;25(2):340-53.
Savary E, Hugnot JP, Chassigneux
Y, Travo C, Duperray C, Van De Water T, Zine A. Distinct
population of hair cell progenitors can be isolated from
the postnatal
mouse cochlea using side population analysis. Stem
Cells. 2007 Feb;25(2):332-9
Deleyrolle L, Marchal-Victorion S, Dromard
C, Fritz V, Saunier M, Sabourin JC, Tran Van Ba C, Privat
A, Hugnot JP.
Exogenous and fibroblast growth factor 2/epidermal growth
factor-regulated
endogenous cytokines regulate neural precursor cell growth
and differentiation.
Stem Cells. 2006 Mar;24(3):748-62.
Marchal-Victorion S, Deleyrolle L, De Weille
J, Saunier M, Dromard C, Sandillon F, Privat A, Hugnot
JP.
The human NTERA2 neural cell line generates neurons on growth
under neural stem cell conditions and exhibits characteristics
of radial glial cells. Mol Cell Neurosci. 2003
Sep;24(1):198-213.
Hugnot JP, Mellodew K,
Pilcher H, Uwanogho D, Price J, Sinden JD.
Direct cell-cell interactions control apoptosis and oligodendrocyte
marker
expression of neuroepithelial cells. J Neurosci Res. 2001
Aug 1;65(3):195-207.
Salinas M, de Weille J, Guillemare E, Lazdunski
M, Hugnot JP.
Modes of regulation of shab K+ channel activity by the Kv8.1 subunit.
J Biol Chem. 1997 Mar 28;272(13):8774-80.
Hugnot JP, Salinas M, Lesage
F, Guillemare E, de Weille J, Heurteaux C, Mattei MG, Lazdunski
M.
Kv8.1, a new neuronal potassium channel subunit with specific inhibitory
properties towards Shab and Shaw channels.
EMBO J. 1996 Jul 1;15(13):3322-31.
Hugnot JP, Gilgenkrantz
H, Vincent N, Chafey P, Morris GE, Monaco AP,
Berwald-Netter Y, Koulakoff A, Kaplan JC, Kahn A, et al.
Distal transcript of the dystrophin gene initiated from an alternative
first exon
and encoding a 75-kDa protein widely distributed in nonmuscle
tissues.
Proc Natl Acad Sci U S A. 1992 Aug
15;89(16):7506-10.
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