The INM histology platform, labeled IBISA since 2015, is part of the experimental histology network of Montpellier (RHEM). Created in 2008, this network is dedicated to the histopathological analysis of experimental models. RHEM has now integrated "BioCampus Montpellier" (BBCM), a structure that federates all the technical resources of the BioSante RABELAIS pole. The INM-RHEM histological platform is open to the scientific community, both public and private. Its skilled personnel offer a wide range of histological protocols and can provide adequate support and training together with the access to high tech equipment.
The RHEM goals are to rationalize human and material resources, expertise and skills for experimental histology. To meet the needs of academic and private users, RHEM offers technical support, equipment and reagents, as well as training and education. The technical expertise and the equipment available within the RHEM network allow the processing of a large variety of tissue samples under adequate security confinement (L1, L2 or L3 Laboratories, secure storage area).
All the projects ran within the RHEM network involve a quality policy and a full traceability of each specimen “from the animal to the picture", in which the RHEM is the link between the “Réseau des Animaleries de Montpellier” (RAM) and “Montpellier RIO Imaging” (MRI).
Within this network, the main plateau of the INM can provide a complete solution from the preparation of biological samples to the analysis, within the same site. The Histology platform at the INM offers to the scientific community a 135m2 laboratory, with a wide range of histological approaches, technical support and the access to quality equipment.
1. Equipment and workstations.
All apparatus and workstations are available on a free access base after a specific training. They can be booked on our website.
1.1. Cutting devices
- Vibratome Microm HM 650 (sections 30 to 500 µm)
- Four Leica cryostat 3050 (Sections 8 to 30 µm)
- Leica RM 2255 (paraffin sections 3 to 10μm)
- Leica Ultracut E (resin embedded specimen, 80nm to 2μm)
1.2. Devices for embedding
- Microwave histos 5 Millestone (decalcification, unmasking, paraffin inclusion ...)
- Paraffin coating station
- Leica EG 1150C (orientation and embedding of samples in paraffin)
- SnapFrost Excilon (rapid freezing to - 80°)
Space is devoted to workstations (prep for photonic or electron microscopy). They are equipped with a Zeiss binocular microscope with optic fibers illumination, Zeiss fluorescence upright microscope.
All chemical and small equipment necessary for the preparation are available: fixatives, buffers, embedding media, dyes, etc. Specific compounds for immunostaining: unmasking solution, amplification kits, mounting media, etc.; secondary antibodies classified by species either fluorescent, coupled with Alexa or non-fluorescent, coupled with peroxidase, can be obtained.
2. Services proposed
2.1. Transparisation X.Clarity : all types of biological samples (reporter genes: tomato, GFP, YFP,... and fluorescence immunolabeling) - Observation two-Photon Zeiss, X.Clarity objective x20 - 3D Imaris imaging
2.2. Supports of scientific project: guidance and counseling
- Fixation by intracardiac perfusion
- Organ harvesting
- Sections (frozen, vibratome, paraffin)
- Development of immunostaining techniques: multi staining in bright field or in fluorescence (on paraffin sections, frozen or floating organotypic cultures, dissociated cells, cell pellets)
- Classic Staining
3. On-demand training, academic or private
On request, future users can be trained either in the practice of different techniques (immunostaining, pre-embedding, etc.) or in the use of a specific equipment.
4. Achievements of the INM-RHEM
- Philipot D., et al., Arthritis Res Ther, 16(1):R58, 2014
- Manes G., et al., Am J Hum Genet, 93(3):571-8, 2013
- Guichet P.O., et al., Glia, 61(2):225-39, 2013
- Lucas O., et al., Mol Cell Neurosci, 50(3-4):211-20, 2012
- Mamaeva D., et al., BMC Neurosci, Oct 10;12:99, 2011
- Courties G., et al., Blood, 116(18):3505-16, 2010