The platform is open to regular or punctual users from the INM and the exterior (researchers, post-docs, doctoral students, students, technical staff) who wish to carry out in situ hybridization and real time PCR.
The manager of the gene expression platform is responsible for:
- organisation and management of the platform
- coordination of the different users
- advice and direction for future users
- training of new users for in situ hybridization (from preparation of probes to labeling tissues) and real time PCR
- development and adaptation of methodology to specific needs
1. In Situ hybridization
Users have all the necessary equipment (incubators, waterbaths, micropipettes, hybridisation oven, fume hood, microscopes to follow the colour development) and consumables at their disposition for carrying out in situ hybridization on whole tissues or section.
The in situ hybridisation laboratory is an RNase-free environment in which users have all the material necessar for extraction of RNA (Polytron, Ultratorax, centrifuges).
- simple in situ hybridization
- 2-colour double in situ hybridization
- hybridization in situ coupled with immunhistochemistry
- microRNA in situ hybridization
- fluorescent double in situ hybridization
EXAMPLES OF LABELING ON DIFFERENT TISSUES STUDIED AT THE INM
Retina Cochlea Spinal cord and Dorsal Root Ganglions
Nerve Double in situ hybridization ISH coupled with immunhistochemistry
2. Real time PCR
Users have all the necessary equipment at their disposition for carrying out real time PCR:
Light Cycler 2.0 32 capillaries Light Cycler 480 plates 96 wells
The DNA free laboratory is an DNA-free environment in which users can do all the mix for PCR without the DNA matrix.
National training provided as part of the Continuing Education of INSERM (2008 and 2012). The next session is programmed for October 2014.
The next session is programmed for October 2014.
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