The platform is open to regular or punctual users from the INM and the exterior (researchers, post-docs, doctoral students, students, technical staff) who wish to carry out in situ hybridization and real time PCR.

The manager of the gene expression platform is responsible for:

- organisation and management of the platform
- coordination of the different users
- advice and direction for future users
- training of new users for in situ hybridization (from preparation of probes to labeling tissues) and real time PCR
- development and adaptation of methodology to specific needs

1. In Situ hybridization

Users have all the necessary equipment (incubators, waterbaths, micropipettes, hybridisation oven, fume hood, microscopes to follow the colour development) and consumables at their disposition for carrying out in situ hybridization on whole tissues or section.

Expression gnique extraction ARNt

 

The in situ hybridisation laboratory is an RNase-free environment in which users have all the material necessar for extraction of RNA (Polytron, Ultratorax, centrifuges).


 

 

 

Available protocols:
    • simple in situ hybridization
    • 2-colour double in situ hybridization
    • hybridization in situ coupled with immunhistochemistry
    • microRNA in situ hybridization
    • fluorescent double in situ hybridization
              

EXAMPLES OF LABELING ON DIFFERENT TISSUES STUDIED AT THE INM

Venteo-retine           venteo-cochlee           venteo-moelle-epiniere

          Retina                                             Cochlea                        Spinal cord and Dorsal Root Ganglions

venteo-nerf          Hybrid 3             Hybrid 6

          Nerve                              Double in situ hybridization       ISH coupled with immunhistochemistry


2. Real time PCR


Users have all the necessary equipment at their disposition for carrying out real time PCR:

                     Light Cycler 2.0 32 capillaries                       Light Cycler 480 plates 96 wells

           Expression gnique LC2.0        Expression gnique LC480

The DNA free laboratory is an DNA-free environment in which users can do all the mix for PCR without the DNA matrix.

3. Education

Poster Formation



National training provided as part of the Continuing Education of INSERM (2008 and 2012). The next session is programmed for October 2014.

The next session is programmed for October 2014.




 



Major publications

Coque E et al., Proc Natl Acad Sci USA pii: 201815961, 2018
Enault S et al., BMC Evol Biol. 30;18(1):127, 2018
Rivat C et al., Nat Commun. 9:1042, 2018
Ventéo S et al., Sci Rep. 6:36407, 2016
Ohayon D et al., Cell Rep. 17:1473-1481, 2016
Bouilloux F et al., Elife Feb 8;5. pii: e11627, 2016
Ohayon D et al., Dev Cell. 33:343-50, 2015
Enault S et al., Front Genet 15;6:283, 2015
Elzière L et al., PLoS ONE 19:e97736, 2014
Ventéo S et al., PlosOne 7(1):e29852, 2012
Wende H et al., Science 335(6074):1373-6, 2012
Dalet A et al., PlosOne 7(9):e46261, 2012
Fasquelle L et al., J Biol Chem 13;286(19):17383-97, 2011
Grimal S et al., Glia 58(16):1977-87, 2010

 

Contact

Ventéo Stéphanie