U583

Team 1
Genetics and therapy of retinal blindness

Team 2
Pathophysiology and therapy of the inner ear

Team 3
Cellular and molecular neurobiology of the somatosensory system

Team 4
Physiology and therapeutic approaches of spinal cord pathologies

Team 5
Physiopathology and therapy of vestibular disorders

U844

Research fields

Team 03:
Cellular and molecular neurobiology
of the somato-sensory system

Models & Techniques:

• Drosophila embryonic peripheral nervous system (PNS) development
• Chick embryo PNS development
• Transgenic mice models of PNS system development and function
• Lesion of the mouse peripheral nerve (transection, crush etc.)

• Primary cultures of adult and embryonic sensory neurons (mice)
• Electrophysiologie (Patch Clamp)
• Videomicroscopy-Calcium imaging on cultured neurons
• Immunohistochemistry and in situ hybridisation (culture and DRG)
• Biochemistry / Molecular Biology
• Transfection (plasmid/siRNA) of primary cultures (single cell electroporation)
• Transfection in vivo (intrathecal injection in mice)
• Electroporation of chick embryo

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