Institut des Neurosciences de Montpellier

U583

Team 1
Genetics and therapy of retinal blindness

Team 2
Pathophysiology and therapy of the inner ear

Team 3
Cellular and molecular neurobiology of the somatosensory system

Team 4
Physiology and therapeutic approaches of spinal cord pathologies

Team 5
Physiopathology and therapy of vestibular disorders

U844

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Research fields

Team 03:
Cellular and molecular neurobiology
of the somato-sensory system

Peripheral Nerve Injury and Regeneration

Head group
Frédérique SCAMPS (CR1 INSERM)
Tel : +33 (0) 499 636 033
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GROUP

·Jean Valmier (PU)
·Michel CHAMMAS (PU-PH)
·Cécile Hilaire (MCU)
·Victor Sieso (MD, MCU)
·Sylvie Mallié (TR)
·Chamroeun Sar (AI)

·Olivier Lucas (doc)

Research Interests
Objectives
Scientific projects
Publications

Research Interests

The peripheral nerve injury is a frequent traumatism that induces a temporary disconnection between muscular fibre and sensory and motor nerve endings, followed by cellular modifications leading to functional regeneration. Unsuccessful regeneration leads to ataxic behaviour and to the development of neuropathic pain.
The chloride homeostasis is under the control of two major families of membrane proteins: chloride channels and co-transporters. Chloride homeostasis is involved in the regulation of cell excitability and volume. The role of chloride homeostasis in the physiology of sensory neurons as well as in the regeneration of the peripheral nerve remains largely unexplored.

Objectives

What is the role of chloride homeostasis in pain and regeneration ? What are the intracellular signalling pathways that regulate chloride homeostasis? What are the subsets of neurons involved in abnormal pain?

Regenerative growth in vitro of an injured sensory neuron

Scientific projects

* Identification and role of mecano-sensitive channels during nerve regeneration

* Translational control of mRNA and nerve regeneration

* Pre-clinical studies for peripheral nerve repair

Publications

Boudes M., Sar C., Menigoz A., Hilaire C., Pequignot M., Kozlenkov V., Marmorstein A., Carroll P., Valmier J., Scamps F. (2009). Best1 is a gene regulated by nerve injury and required for Ca2+ -activated C1- current expression in sensory neurons in mice. J. Neurosci. 29:10063-71.

Boudes, M., Pieraut, S., Valmier, J., Carroll, P. and Scamps, F. (2008). Single-cell electroporation of adult sensory neurons for gene screening with RNA interference mechanism. J. Neurosci. Methods 170:204-11.

Pieraut, S., Matha, V.,Sar, C., Hubert, T., Méchaly, I., Hilaire, C., Mersel, M., Delpire, E., Valmier, J. and Scamps, F. (2007). NKCC1 phosphorylation stimulates neurite growth of injured adult sensory neurons. J. Neurosci. 27 : 6751-6759.

Aptel, H., Hilaire, C., Pieraut, S., Valmier, J., Scamps, F. (2007) T-type Ca2+ current is a molecular determinant of excitatory effects of GABA in adult sensory neurons. Mol.Cell.Neurosci.36: 293-303.

Hilaire, C., Campo, B., André, S., Valmier, J. and Scamps, F. (2005) K+ current regulates calcium-activated chloride current-induced afterdepolarization in axotomized sensory neurons. Eur. J. Neurosci. 22:1073-1080.

Dubreuil, AS, Boukhaddaoui, H., Desmadryl, G., Martinez-Salgado, C., Lewin, G.R., Carroll, P., Valmier, J. and Scamps, F.(2004). Role of T-type calcium current in identified D-hair mechanoreceptor neurons studied in vitro. J. Neurosci. 24: 8480-8484.

André, S., Boukhaddaoui, H., Campo, B., Al-Jumaily, M., Mayeux, V., Greuet, D., Valmier, J. and Scamps, F. (2003). Axotomy-induced expression of calcium-activated chloride current in subpopulations of mouse dorsal root ganglion neurons. J. Neurophysiol.90 : 3764-3773.

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