Team :

JP Hugnot, Assistant Professor, Montpellier 2 University, group leader
L Bauchet, Neurosurgeon, Gui de Chaulliac Hospital
H Guillon, Post doctoral, researcher
JC Sabourin, PhD student
PO Guichet , PhD student

Jean Philippe Hugnot	Luc Bauchet	Hélène Guillon	Jean-Charles Sabourin	Pierre-Olivier Guichet

The adult nervous system contains an heterogeneous population of immature cells referred to as neural stem and progenitor cells. These endogenous neural precursors are of great interest for therapeutic purpose as they could theorically compensated for lost cells in traumatic injury and neurodegenerative & demyelinating diseases. In addition, transplantation of these cells in animals and in man have shown that these cells could improve clinical deficits associated to various diseases. The diversity and the differentiation control of these cells remain to be fully explored.
We are studying neural stem cells in vivo and in vitro in mice and in Human. Our work aims at:
1/ Identify and explore the function and the plasticity of neural precursor cells in Human and mouse spinal cord.

2/ Analyse the molecular mechanisms underlying the self renewal and differentiation of neural stem cells in physiological and pathological situations (glioma and spinal cord trauma)

Figure 1 : The neural lineage (simplified)

Figure 2 : Neurospheres derived from the adult human spinal cord.

Figure 3: Neurosphere differentiation in 3 cell types: Astrocytes (green), neurons (red) and oligodendrocytes (blue).

Publications

1: Sabourin JC, Ackema KB, Ohayon D, Guichet PO, Perrin FE, Garces A, Ripoll C, Charité J, Simonneau L, Kettenmann H, Zine A, Privat A, Valmier J, Pattyn A, Hugnot JP. A mesenchymal-like ZEB1(+) niche harbors dorsal radial glial fibrillary acidic protein-positive stem cells in the spinal cord. Stem Cells. 2009 Nov;27(11):2722-33. 

2: Sanz LA, Chamberlain S, Sabourin JC, Henckel A, Magnuson T, Hugnot JP, Feil R, Arnaud P. A mono-allelic bivalent chromatin domain controls tissue-specific imprinting at Grb10. EMBO J. 2008 Oct 8;27(19):2523-32.

3: Dromard C, Guillon H, Rigau V, Ripoll C, Sabourin JC, Perrin FE, Scamps F, Bozza S, Sabatier P, Lonjon N, Duffau H, Vachiery-Lahaye F, Prieto M, Tran Van Ba C, Deleyrolle L, Boularan A, Langley K, Gaviria M, Privat A, Hugnot JP, Bauchet L. Adult human spinal cord harbors neural precursor cells that generate neurons and glial cells in vitro. J Neurosci Res. 2008 Jul;86(9):1916-26.

4: Armando S, Lebrun A, Hugnot JP, Ripoll C, Saunier M, Simonneau L. Neurosphere-derived neural cells show region-specific behaviour in vitro. Neuroreport. 2007 Oct 8;18(15):1539-42.

5: Hachem S, Laurenson AS, Hugnot JP, Legraverend C. Expression of S100B during embryonic development of the mouse cerebellum. BMC Dev Biol. 2007 Mar 15;7:17.

6: Adult human spinal cord harbours neural precursor cells that generate neurons and glial cells in vitro. C. Dromard, H. Guillon, V. Rigau, C. Ripoll, S Bozza, P. Sabatier, N Lonjon, H Duffau, F. Vachiery-Lahaye, M. Prieto, C Tran Van Ba, L. Deleyrolle, A. Boularan, K. Langley, M. Gaviria, A. Privat, L. Bauchet*, J.P. Hugnot*, J Neurosci Res, 2007, in press (*= co auteurs)

7: Bauchet L, Rigau V, Mathieu-Daudé H, Figarella-Branger D, Hugues D, Palusseau L, Bauchet F, Fabbro M, Campello C, Capelle L, Durand A, Trétarre B, Frappaz D,Henin D, Menei P, Honnorat J, Segnarbieux F. French brain tumor data bank: methodology and first results on 10,000 cases.J Neurooncol. 2007 Sep;84(2):189-99.

8: Dromard C, Bartolami S, Deleyrolle L, Takebayashi H, Ripoll C, Simonneau L,Prome S, Puech S, Tran Van Ba C, Duperray C, Valmier J, Privat A, Hugnot JP. NG2 and olig2 expression provides evidence for phenotypic deregulation of cultured CNS and PNS neural precursor cells. Stem Cells. 2006 Oct 19; [Epub ahead of print]

9: Savary E, Hugnot JP, Chassigneux Y, Travo C, Duperray C, Van De Water T, Zine A.
Distinct population of hair cell progenitors can be isolated from the postnatal mouse cochlea using side population analysis. Stem Cells. 2006 Oct 12; [Epub ahead of print]

10: Deleyrolle L, Marchal-Victorion S, Dromard C, Fritz V, Saunier M, Sabourin JC, Tran Van Ba C, Privat A, Hugnot JP. Exogenous and fibroblast growth factor 2/epidermal growth factor-regulated endogenous cytokines regulate neural precursor cell growth and differentiation. Stem Cells. 2006 Mar;24(3):748-62. Epub 2005 Sep 15.

11: Marchal-Victorion S, Deleyrolle L, De Weille J, Saunier M, Dromard C, Sandillon F, Privat A, Hugnot JP. The human NTERA2 neural cell line generates neurons on growth under neural stem cell conditions and exhibits characteristics of radial glial cells. Mol Cell Neurosci. 2003 Sep;24(1):198-213.

12: Hugnot JP, Mellodew K, Pilcher H, Uwanogho D, Price J, Sinden JD. Direct cell-cell interactions control apoptosis and oligodendrocyte marker expression of neuroepithelial cells. J Neurosci Res. 2001 Aug 1;65(3):195-207.

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