HISTOLOGY Facility INM (immunocytochemistry)

Head : Chantal RIPOLL

The immunocytochemistry section of the histology facility was raised to welcome all INM users (researchers, students) as well as people from outside INM to the limits of available space, who wish to perform histological or immunocytochemical investigations, on either a steady or occasional basis.

Open for all users :
- usual buffers
- fluorescent Alexa secondary antibodies
- small precision equipment (binocular magnifying glasses, balances, incubators)

MICROM vibratome with its cooled chamber

The facility harbors several vibratomes, whose the most recent is cooled, allowing to obtain 20- to 50 µm-thick slices, and up to 100 µm, of fresh tissues.

SAMBA station for image analysis
This analysis system is coupled to a fluorescence Zeiss microscope on light background, to perform automatic measures in density and cell count.

Western Blot

The histology facility provides users with the equipment for Western blotting. This is also useful to check for the specificity and affinity of the antibodies used on tissues.

Flat preparation of inner ear sensory cells

Inner hair cell, cryostat section

Drosophila embryo

Vestibular hair cells

Base of a vestibular calix

Double labelled neurons revealed
with immunoperoxidase

Section of neurons in paraffin was stained with cresyl violet