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Technical platforms
The INM houses several technical
facilities which are essential for the development of research.
Most of these services - cell imaging, neurogenetics and the
animal facility - are part of technical networks with the
other montpellierans centers (IGMM, IGH, IGF) to offer a large
panel of techniques to investigators.
1 Cell imaging
Supervision: H Boukhaddaoui
Since its creation in 2004, the INM cell
imaging facility belongs to the Cell Imaging Montpellier Service
which is part of the RIO National Network (www.mri.cnrs.fr).
The INM cell imaging facility is dedicated to cell imaging
in neurosciences. It offers to registered users a panel of
highly sophisticated devices in photonic microscopy. The facility
is available to the scientific community 24 hours / day, 7
days / week and 52 weeks / year.
Equipments :
 Two-photon
microscope: especially equipped to record fast events
occurring during the transfer of sensory information in explant
cultures or sections.
Confocal
microscope: rebuilding 3D on a sub cellular scale.
Automated
Videomicroscope: allowing the observation of cellular
mechanisms over a long period of time, in particular during
neuronal differentiation and cell death.
Dynamic
Videomicroscope: to follow ionic movements.
Videomicroscopes
CCD camera: 4 instruments for fluorescence microscopy.
Station
for image analysis: (Metamorph, lasersharp, 2D analysis,
metamorph laserpix, Imaris laservox for the 3D analysis and
rebuilding).
Projects :
Two important innovative technological projects
are currently in negotiation at the INM cell imaging facility
in cooperation with the Cell Imaging Montpellier RIO Service.
(i) We need a DUO confocal device, especially equipped with
2 scanning systems. One is a relatively slow conventional
system to perform multidimensional imaging on fixed tissues.
The other is a fast scanning system (120 images/sec) to study
unfixed tissues. It is adapted for the observation of the
fast dynamic processes on delicate alive preparations such
as nerve cells.
(ii) We also need a high throughput imaging automated system
entirely dedicated to immuno-histological investigations (see
color images). This tool is indispensable to toxicity studies
and to high rate screenings. It will be located at the INM
histology facility.
Currently, such equipments are not available
in Montpellier. These two systems will be integrated into
the Cell Imaging Montpellier RIO Service and will thus be
offered to the whole public and private scientific community.
All users can benefit from the INM cell imaging facility as
long as they pay the hourly cost in definite conditions. www.mri.cnrs.fr
(560 users in 2006).
2 Neurogenetics
Supervision: Audrey Sénéchal
3 Histology
Supervision: Chantal Ripoll
This service is divided into 2 sections:
Microtomy
Immunocytochemistry
4 Electrophysiology:
INM has 8 patch clamp and in-vivo recording
stations, including 2 stations coupled to fluorescence imaging.
5
Functional Investigation Platform
Supervision: Philippe Alzieu
6
Platform ELISA
Supervision: Pene Jérôme et Courties Gabriel
7
Platform FACS
Supervision: Pene Jérôme et Plence Pascale
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