Contract : Fixed-term contract, post-doctoral fellowship
Duration : 36 months
Funding is provided by the ANR. Brut salary from 2555 € / month (Inserm salary grid) depending on experience.
Beginning : 01/09/2021
Laboratory : Institute for Neurosciences of Montpellier
Mutations in large genes are a frequent cause of inherited retinal diseases, and can result in severe visual impairment. However, as the causative genes exceed the cloning capacity of the most currently used viral vector to date, developing a gene replacement therapy is challenging. As part of a European network of 7 laboratories, we aim to meet this challenge and develop an alternative therapeutic strategy for large disease-causing genes in human retinal organoids derived from patient induced pluripotent stem cells (iPSCs).
The applicant will join the “Gene therapy of inherited retinal dystrophies” group within the Vision team of the INM (http://www.inmfrance.com/inmfrance-j3/index.php/en/vision-en). This dynamic group is interested in developing innovative strategies to treat genetic retinal diseases.
Mamaeva D, Jazouli Z, DiFrancesco ML, Erkilic N, Dubois G, Hilaire C, Meunier I, Boukhaddaoui H & Kalatzis V. (2021) Novel roles for voltage-gated T-type Ca2+ and ClC-2 channels in phagocytosis and angiogenic factor balance identified in human iPSC-derived RPE. FASEB J. 35:e21406.
Diakatou M, Dubois, G, Erkilic N, Sanjurjo-Soriano C, Meunier I & Kalatzis V. (2021) Allele-specific knockout by CRISPR/Cas to treat autosomal dominant retinitis pigmentosa caused by the G56R mutation in NR2E3. Int. J. Mol. Sci. 20: 2542.
Sanjurjo-Soriano C, Erkilic N, Baux D, Mamaeva D, Hamel CP, Meunier I, Roux AF & Kalatzis V. (2019) Genome Editing in Patient iPSCs Corrects the Most Prevalent USH2A Mutations and Reveals Intriguing Mutant mRNA Expression Profiles. Mol. Ther. Methods Clin. Dev. 17:156-173.
Torriano S, Erkilic N, Faugère V, Damodar K, Hamel CP, Roux A-F & Kalatzis V. (2017) Pathogenicity of a novel missense variant associated with choroideremia and its impact on gene replacement therapy. Hum. Mol. Genet. 26:3573-3584.
- Conduct cell culture experiments (iPSC culture, differentiation into retinal organoids)
- Conduct cell biology experiments (cryosections, immunofluorescence, immunoblot)
- Conduct molecular biology experiments (RNA/DNA extraction, qPCR, cloning techniques)
- Record and format results
- Communicate experimental data
- Control the disposal of solid waste and effluents according to the health and safety rules of the field
- General knowledge of cell culture and cellular and molecular biology
- Knowledge of health and safety rules related to work in confinement
- Knowledge of English: oral level B2, read and written level B2
- Master the techniques of manipulation in cell culture.
- Master the techniques of molecular biology and the use of associated equipment
- Evaluate the efficiency of the protocols used and make them evolve
- Use computer software to analyze and format experimental data experimental data
- Write technical procedures, transmit techniques and know-how
- Work in a team
- Have very good organizational skills and flexibility
- Rigor in technical practice and archiving
- Experience in cell culture and molecular biology
- Experience in gene therapy is a plus
- Work in a containment laboratory
- Occasional weekend work